This antibody can be used in ELISA (1:5,000 dilution), Western blot (1:1,000 dilution), and indirect immunofluorescence (1:500). It recognizes a 31 kDa protein corresponding to the structural VP3 protein. IPNV is a single-shelled, non-enveloped virus that belongs to the family Birnaviridae. Its genoma comprises a bisegmented double-stranded RNA, the segment B encodes VP1 and the segment A yields a polyprotein, pVP2-VP4-VP3 and a small protein (VP5) dispensable for viral replication. The polyprotein is autocatalytically cleaved by the endoprotease VP4 to release the structural proteins VP3 and pVP2. VP3 is an internal protein that interacts with both VP1 and dsRNA in a mutually independent mannner. IPNV is responsible for infectious pancreatic necrosis, a contagious fish disease characterized by severe damage to the internal organs and tissues with a high mortality among salmonids.

Catalog Number: FM-130DZ-5.

Product Name: Anti-Infectious Pancreatic Necrosis Virus (IPNV) VP3 protein, IgG fraction monoclonal antibody (clone 6E1/F2).

Storage Temperature: -20ºC.

Unit: 100µg.

Category: Infectious Agents.

Subcategory: Fish Pathogens (Aquaculture).

Description:
Mouse monoclonal antibody IgG fraction (clone 6E1/F2) obtained by immunizing mice with purified IPN virus. The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG1.

Mol Weight: N/A.

Purity: N/A.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Formulation: Solution at 0.5 mg/mL in PBS.

References:

1. Pedersen, T. et al. (2007) J. Virol. 81, 6652-6663.

2. Roberts, R. J. & Pearson MD. (2005) J. Fish Dis. 28, 383-390.

3. Rodriguez Saint-Jean S. et al. (2003) 62, 113-165.

4. Duncan, R. et al. (1991) Virology 181, 541-552.