This antibody can be used in ELISA (1:500 dilution), Western blot (1:1,000 dilution), and indirect immunofluorescence (1:1,000). It recognizes two bands, one at 42 kDa corresponding to ISAV hemagglutinin-esterase and the second at 90 kDa probably corresponding to a dimmer of this protein. ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage.

Catalog Number: FM-122AX-5.

Product Name: Anti-Infectious salmon anemia virus (ISAV) Hemagglutinin-Esterease protein, IgG fraction monoclonal antibody (clone 8D2/E9).

Storage Temperature: -20ºC.

Unit: 100µg.

Category: Infectious Agents.

Subcategory: Fish Pathogens (Aquaculture).

Description:
Mouse monoclonal antibody IgG fraction (clone 8D2/E9) obtained by immunizing mice with a purified N-terminal hemagglutinin recombinant fragment of ISAV. The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG2b.

Mol Weight: N/A.

Purity: N/A.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Formulation: Solution at 1.0 mg/mL in PBS.

References:

1. Krossby, B. et al. (2001) J. Gen. Virol. 82, 1757-1765.

2. Rimstad, E. & Mjaaland, S. (2002) APMIS 110, 273-282.

3. Falk,K. et al. (2004) J. Virol. 78, 3063-3071.

4. Aspehaug, V. et al. (2004) Virus Res. 106, 51-60.

5. Goic, B. et al. (2008) Virology 379, 55-63.