This antibody can be used in ELISA (1:1,000 dilution), Western blot (1:1,000 dilution), and indirect immunofluorescence (1:500). It recognizes a 50 kDa protein corresponding to ISAV fusion protein. ISAV is an enveloped virus that belongs to the family Orthomyxoviridae and genus Isavirus. Its genoma comprises eight negative-sense, single-stranded RNA segments and encodes four major structural proteins including the matrix, the nucleoprotein, and two membrane glycoproteins (hemagglutinin-esterase and fusion protein gp50). ISAV infects mainly endothelial cells and leukocytes and causes a multisystemic disease characterized by high mortality with exophthalmia, pale gills, ascites, hemorrhagic liver necrosis, and renal interstitial hemorrhage.

Catalog Number: FM-123AY-5.

Product Name: Anti-Infectious salmon anemia virus (ISAV) Fusion protein, IgG fraction monoclonal antibody (clone 8B2/A4).

Storage Temperature: -20ºC.

Unit: 100µg.

Category: Infectious Agents.

Subcategory: Fish Pathogens (Aquaculture).

Description:
Mouse monoclonal antibody IgG fraction (clone 8B2/A4) obtained by immunizing mice with a purified N-terminal fusion ISAV protein recombinant fragment. The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG2a.

Mol Weight: N/A.

Purity: N/A.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Formulation: Solution at 1.0 mg/mL in PBS.

References:

1. Rimstad, E. & Mjaaland, S. (2002) APMIS 110, 273-282.

2. Ritchie, R.J. et al. (2002) Virus Res. 84, 161-170.

3. Falk,K. et al. (2004) J. Virol. 78, 3063-3071.

4. Kibenge, F.S. et al. (2007) 4, 34.

5. Goic, B. et al. (2008) Virology 379, 55-63.